The MassFluidix HC – Sample Prep Kit is designed to streamline and enhance your mass photometry experiments by integrating fast sample dilution and measurement into a single workflow. Specifically developed for use with Refeyn’s MassFluidix HC accesory for mass photometry instruments, this kit enables precise data acquisition.
Each kit includes:
The MassFluidix HC chip is a microfluidic consumable that combines sample and dilution buffer using a reverse Tesla valve mixer, allowing for very fast dilution immediately prior to starting the experiment – less than 37 ms from the sample inlet to the observation area. Once mixed, the diluted sample flows through a defined observation area where the mass photometry measurement is performed. This workflow effectively increases the concentration range of Refeyn mass photometers up to 50 µM, enabling the analysis of low affinity interactions.
In high-concentration workflows, the MassFluidix HC system extends the power of mass photometry to samples up to 50 micromolar. It allows researchers to study protein-protein interactions, binding behaviour, and oligomerization dynamics in conditions closer to physiological relevance.
Please refer to page number 25 in the linked user manual for further details.
| Pack Size | 1 |
|---|---|
| Contents | • 1 MassFluidix HC chip (1 chip/pack, 5 measurements/chip) • 2 x bag of mini leur connectors (10/bag) • 1 box of lens Cleaning Tissues (25 sheets) |
| Mass Range | 50 kDa – 5 MDa |
| Number of Wells | 5 channels |
| Number of Experiments Conducted | 5 samples |
| Reusable | No |
| Instrument Compatibility | MassFluidix HC |
| Sample Compatibility | Proteins |
| Storage Instructions | Room Temperature, in a clean environment |
| Shipment Conditions | Room temperature |
| Shelf Life | 6 months |
| Shelf Life After Opening | 10 days |
MassFerence P1 enables accurate, reproducible protein mass calibration for all MP systems, including OneMP, TwoMP, TwoMP Auto and MyMassMP.
MassFluidix HC Preparation Plate simplifies chip setup with fast, error-free connections while protecting the objective.
MassGlass UC Sample Prep Kits enable reliable AAV and protein analysis on SamuxMP and TwoMP with clean, ready-to-use slides for reproducible results.
MP Alignment Kit streamlines AAV and protein analysis with SamuxMP and TwoMP for precise, reproducible results.
Can I store a MassFluidix HC chip with some unused channels for later use?
Yes, since you can partially use a MassFluidix HC chip, you can store it with the remaining unused channels for later use. Once used and cleaned of any remaining immersion oil, the MassFluidix HC chip can be stored in its plastic box, ready to use the remaining channels. Indicate which channels have already been used, and remember the MassFluidix HC chip should be used within 10 days after opening for best results.
Can I re-use a channel?
No, each channel is single-use only to guarantee optimal results and avoid any sample or buffer cross-contamination, ensuring the best data quality is always obtained.
When using the MassFluidix HC, should I calibrate on a MassFluidix HC chip?
No, when conducting a MassFluidix HC experiment, Refeyn advises calibrating in the usual way, utilizing the TwoMP system, on a MassGlass UC carrier slide.
Are the MassFluidix HC chip surfaces similar to the MassGlass UC slides?
Yes, the MassFluidix HC chips are based on MassGlass UC sample carrier slides.
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